Journal: Molecular Pharmaceutics

Article Title: Non-Invasive Detection of Passively Targeted Poly(ethylene glycol) Nanocarriers in Tumors

doi: 10.1021/mp2003913

Figure Lengend Snippet: Passive tumor distribution of PEG nanocarriers measured non-invasively using a SkinSkan® spectrofluorometer. Nanocarriers (0.5 mM) were intravenously administered to 4T1 tumor-bearing balb/c mice and fluorescence spectra were collected from tumor and a contralateral skin (λexc.: 480 nm; λem.: 515 nm for 10–30 kDa and 520 nm for 40–60 kDa). Each column and error bar represents the mean±SD (n = 5–7). Individual comparisons between the groups were determined by Student’s t-test using the Microsoft Excel 2008. *: p<0.05, tumor vs. corresponding dermal control site.

Article Snippet: The 4T1 murine breast cancer cell line was a generous gift from Dr. Michael Reiss, The Cancer Institute of New Jersey (New Brunswick, NJ).

Techniques: Fluorescence, Control

Journal: Molecular Pharmaceutics

Article Title: Non-Invasive Detection of Passively Targeted Poly(ethylene glycol) Nanocarriers in Tumors

doi: 10.1021/mp2003913

Figure Lengend Snippet: (A) Non-invasive images showing the passive tumor distribution of PEG nanocarriers. The images were obtained on an IVIS® 100 imaging system using the excitation and emission filters corresponding to green fluorescent protein (GFP). The first two animals (balb/c mice with 4T1 tumor), starting from the left, were untreated (controls), whereas the remaining three animals were administered nanocarriers (0.5 mM) intravenously; (B) Regions of interest for tumor and control areas are shown in a mouse injected with 40 kDa nanocarrier for average efficient quantitation; and (C) Plots of average fluorescence from tumor and control site at skin’s surface against time. Each point represents the mean±SD (n = 3). Individual comparisons between groups were determined by Student’s t-test using the Microsoft Excel 2008. *: p<0.01, tumor vs. corresponding control site.

Article Snippet: The 4T1 murine breast cancer cell line was a generous gift from Dr. Michael Reiss, The Cancer Institute of New Jersey (New Brunswick, NJ).

Techniques: Imaging, Control, Injection, Quantitation Assay, Fluorescence

Journal: Molecular Pharmaceutics

Article Title: Non-Invasive Detection of Passively Targeted Poly(ethylene glycol) Nanocarriers in Tumors

doi: 10.1021/mp2003913

Figure Lengend Snippet: Ex vivo distribution studies: (A) Tissue distribution at 24 hrs; (B) Tumor distribution at 24 and 96 hrs; and (C) Plasma distribution at 24 and 96 hrs. PEG nanocarriers (0.5 mM) were intravenously administered to balb/c mice bearing 4T1 tumors and animals were euthanized to collect tissues. Each column and error bar represents the mean±SD (n = 5–7). The statistical analyses were carried out using GraphPad Prism v.4 as follows: (A) Two-way ANOVA and individual comparison between the groups were determined using Bonferroni posttests. The statistically significant groups (p<0.05, 60 kDa vs. other nanocarriers) are marked as * (10 kDa); & (20 kDa); # (30 kDa); and + (40 kDa); (B) One-way ANOVA and individual comparison between the groups were determined using Tukey posttests. The statistically significant groups (p<0.05, 60 kDa vs. other nanocarriers are marked as * (10, 20 or 30 kDa); and # (40 kDa at 96 hrs); (C) One-way ANOVA and comparison between the groups were determined using Tukey posttests. The statistically significant groups (p<0.05, 60 kDa vs. other nanocarriers) are denoted by * (10 or 20 kDa); & (30 kDa); # (40 kDa); and + (nanocarriers at 96 hrs).

Article Snippet: The 4T1 murine breast cancer cell line was a generous gift from Dr. Michael Reiss, The Cancer Institute of New Jersey (New Brunswick, NJ).

Techniques: Ex Vivo, Clinical Proteomics, Comparison